The monastery is dedicated to St. Anthony the Great, the father of monasticism, the renowned 3rd century anchorite. There are chapels dedicated to Saints Seraphim of Sarov, Demetrios of Thessalonica, John the Baptist, George the Great Martyr, Nicholas the Wonderworker, and Panteleimon the Healer. The main church is dedicated to Saints Anthony and Nectarios the Wonderworker.

The monastery follows the coenobitic rule of monastic life: a brotherhood of monks and novices holding all things in common follow a daily schedule of prayer and work under obedience to the abbot, their spiritual father. The monks’ daily program begins at midnight with personal prayer time and spiritual reading, followed by the cycle of morning prayers and the Divine Liturgy. After a light breakfast and a rest period, the monks begin their work day, attending to prayer and their tasks till evening. Tasks include, among others, construction, groundskeeping, vinedressing, gardening, woodworking, publishing, food preparation, and offering hospitality. The day ends with evening Vespers followed by dinner and Compline.

St. Anthony’s welcomes all visitors. Orthodox men and women visit for a few days of spiritual growth and quietude. Pilgrims may be accommodated at the monastery’s guest facilities. Day visitors can view the chapels and walk the grounds between 10:30 am and 4:00 pm daily. All guests are to be appropriately and modestly dressed. Men are asked to wear long pants and long-sleeved shirts. Women are to wear long skirts (well below the knee), long-sleeved blouses, and scarves. A limited supply of clothing is available at the bookstore. Upon entering the monastery, all visitors are asked to stop at the gate house. Smoking within the monastery grounds is strictly forbidden. Photographs of the buildings, the grounds, and the church interiors are allowed. Visitors may not photograph monks or overnight pilgrims.